PCK1 anticorps (Internal Region)

N° du produit ABIN452441

L’anticorps anti-PCK1 Polyclonal Chèvre est utilisé pour la détection de PCK1 dans des échantillons de Humain, Souris, Rat et Chien. Il a été validé pour IHC (p) et EIA.

Aperçu rapide pour PCK1 anticorps (Internal Region) (ABIN452441)

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PCK1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-PCK1 anticorps

Épitope: Internal Region

Specificité: Recognizes PCK1/PEPCKC (internal).

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-HVNWFRKDKEGK, from the internal region of the protein sequence according to NP_002582.3.

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/8000. Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Cerebral Cortex showsstaining of cytoplasm in pyramidal cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur PCK1

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Autre désignation: PCK1 / PEPCK1

Sujet: PCK1 is a main control point for the regulation of gluconeogenesis. This cytosolic enzyme, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of the corresponding gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme has also been characterized.Synonyms: PEP carboxykinase, PEPCK-C, PEPCKC, Phosphoenolpyruvate carboxykinase cytosolic [GTP], Phosphoenolpyruvate carboxylase

ID gène: 5105

NCBI Accession: NP_002582

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis