DNAJB1 anticorps

N° du produit ABIN453940

Cet anticorps Souris Monoclonal détecte spécifiquement DNAJB1 dans WB, IP, IF, EIA et IHC (fro). Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour DNAJB1 anticorps (ABIN453940)

Antigène: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DNAJB1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 3B9-E6

Détail du produit anti-DNAJB1 anticorps

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Rat

Attributs du produit: Synonyms: Heat shock 40 kDa protein 1, HSP-40, DnaJ protein homolog 1, HDJ-1, DNAJ1, HDJ1, HSPF1,DnaJ homolog subfamily B member 1

Purification: Protein G affinity chromatography

Immunogène: Recombinant protein HSP40 (HDJ1)

Isotype: IgG1

Information d'application

Indications d'application: Western blot. Immunoprecipitation. Immunohistochemistry on frozen sections. ELISA. Immunflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur DNAJB1

Antigène: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Autre désignation: DNAJB1 / HSP40

Sujet: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).Synonyms: DNAJ1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, HDJ-1, HDJ1, HSP-40, HSPF1, Heat shock 40 kDa protein 1

ID gène: 3337

UniProt: P25685