ICAM5 anticorps (C-Term)

N° du produit ABIN453983

Détail du produit anti-ICAM5 anticorps

Antigène: ICAM5 (Intercellular Adhesion Molecule 5 (ICAM5))

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ICAM5 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody recognizes ICAM5 precursor.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-ESPAEGEVFAIQ, from the C Terminus of the protein sequence according to NP_003250.2.

Information d'application

Indications d'application: Peptide ELISA: Antibody Detection Limit Dilution: 1/128000. Western Blot: Preliminary experiments gave an approx 200-250 kDa band in lysates ofHuman Brain (Frontal Cortex and Hippocampus) and Mouse Brain after 0.05 μg/mLantibody staining. Please note that currently we cannot find an explanation in the literaturefor the band we observe given the calculated size of 97.0 kDa according to NP_003250.2. The 200-250 kDa band was successfully blocked by incubation with the immunizingpeptide. Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Brain (Cerebral Cortex)shows axon/dendrite staining leaving cell bodies relatively unstained.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur ICAM5

Antigène: ICAM5 (Intercellular Adhesion Molecule 5 (ICAM5))

Autre désignation: ICAM5 (ICAM5 Produits)

Synonymes: anticorps TLCN, anticorps TLN, anticorps CD50, anticorps ICAM-3, anticorps Icam3, anticorps Tlcn, anticorps ICAM5, anticorps icam5, anticorps tlcn, anticorps tln, anticorps intercellular adhesion molecule 5, anticorps intercellular adhesion molecule 5, telencephalin, anticorps intercellular adhesion molecule 5 L homeolog, anticorps ICAM5, anticorps Icam5, anticorps LOC100511183, anticorps icam5.L

Sujet: ICAM5 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases.Synonyms: ICAM-5, Intercellular Adhesion Molecule 5, TLCN, TLN, Telencephalin

ID gène: 7087

NCBI Accession: NP_003250

UniProt: Q9UMF0

Pathways: Cell-Cell Junction Organization, ER-Nucleus Signaling, Maintenance of Protein Location