beta Catenin anticorps

N° du produit ABIN457419

Cet anticorps anti-beta Catenin est un anticorps Souris Monoclonal détectant beta Catenin dans WB, IP, ICC et ICFC. Adapté pour Humain, Souris et Hamster.

Aperçu rapide pour beta Catenin anticorps (ABIN457419)

Antigène: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivité: Humain, Souris, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Catenin est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Clone: EM-22

Détail du produit anti-beta Catenin anticorps

Fonction: Anti-beta-Catenin Purified

Specificité: The mouse monoclonal antibody EM-22 reacts with C-terminal part of beta-catenin (intracellular antigen), an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.

Réactivité croisée (Details): Human, Mouse, Hamster

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Recombinant human beta-catenin

Isotype: IgG2a

Information d'application

Indications d'application: Immunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line.
Western blotting: Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line, KW1 murine cell line, C57MG murine cell line, 3T3 murine fibroblast cell line.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Détails sur beta Catenin

Antigène: beta Catenin (CATNB) (Catenin, beta (CATNB))

Autre désignation: beta-Catenin

Sujet: Catenin beta 1,Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.,CTNNB1, EVR7, MRD19

ID gène: 1499

UniProt: P35222

Pathways: Peptide Hormone Metabolism