Albumin anticorps

N° du produit ABIN457573

Cet anticorps Lapin Polyclonal détecte spécifiquement Albumin dans ELISA, IEP, ID, Neph et RID. Il présente une réactivité envers Boeuf (Vache).

Aperçu rapide pour Albumin anticorps (ABIN457573)

Antigène: Albumin (ALB)

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Albumin est non-conjugé

Application: ELISA, Immunoelectrophoresis (IEP), Immunodiffusion (ID), Nephelometry (Neph), Radial Immunodiffusion (RID)

Détail du produit anti-Albumin anticorps

Specificité: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in double radial immunodiffusion with the following results: cat + chicken - dog + duck - goat ++ guinea pig ± hamster + horse + human + monkey + mouse ± pigeon - rabbit - rat ± sheep ++ swine +

Attributs du produit: Precipitating polyclonal rabbit antiserum to bovine serum albumin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogène: Highly purified albumin isolated from bovine serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: As precipitating antiserum to identify or measure bovine albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:64 when tested against normal bovine serum in agar block immunodiffusion titr

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Détails sur Albumin

Antigène: Albumin (ALB)

Autre désignation: Serum Albumin

Sujet: The defined antibody specificity is directed to albumin as tested against bovine sera. In immuno- electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against bovine serum and the purified albumin.

Pathways: Lipid Metabolism