Fibrinogen anticorps (FITC)

N° du produit ABIN457655

L’anticorps anti-Fibrinogen Polyclonal Chèvre est utilisé pour la détection de Fibrinogen dans des échantillons de Chien. Il a été validé pour ELISA, IHC (fro), ICC et IFA.

Aperçu rapide pour Fibrinogen anticorps (FITC) (ABIN457655)

Antigène: Fibrinogen

Reactivité: Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibrinogen est conjugé à/à la FITC

Application: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Indirect Immunofluorescence Assay (IFA)

Détail du produit anti-Fibrinogen anticorps (FITC)

Fonction: Goat anti Dog fibrinogen, conjugated with FITC

Specificité: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Dog fibrinogen

Réactivité croisée (Details): The antiSerum does not cross-react with any other component of Dog plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal dog plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As reagent for the direct detection of fibrinogen in dog cells, tissues and body fluids in immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogène: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.

Buffer: Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C.

Détails sur Fibrinogen

Antigène: Fibrinogen

UniProt: P68213, P02677, P12800