Albumin anticorps (HRP)

N° du produit ABIN457983

Détail du produit anti-Albumin anticorps (HRP)

Antigène: Albumin (ALB)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Albumin est conjugé à/à la HRP

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificité: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins frequently share antigenic determinants. of this antiserum has not been tested in detail.

Attributs du produit: Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to human albumin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate the antibody activity to any other component of the human serum proteins. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogène: Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from human serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in human serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:10,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Enzyme/IgG protein molar ratio (E/P) is approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).

Buffer: Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml ster ile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Détails sur Albumin

Antigène: Albumin (ALB)

Autre désignation: Albumin (ALB Produits)

Synonymes: anticorps PRO0883, anticorps PRO0903, anticorps PRO1341, anticorps ALB, anticorps CSA, anticorps Alb-1, anticorps Alb1, anticorps Albza, anticorps LOC100136344, anticorps alb-a, anticorps alb-b, anticorps albumin, anticorps serum albumin 1, anticorps albumin S homeolog, anticorps ALB, anticorps Alb, anticorps LOC100136344, anticorps alb.S

Sujet: Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal human serum and purified human albumin. One characteristic precipitin line is obtained against pooled normal human serum using different antigen/antibody concentration ratio’s. Precipitin lines against normal human serum and purified human albumin give a reaction of full identity

Pathways: Lipid Metabolism