LALBA anticorps (TRITC)
Aperçu rapide pour LALBA anticorps (TRITC) (ABIN458122)
Antigène
Voir toutes LALBA AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Fonction
- Rabbit anti Human alpha lactalbumin, conjugated with TRITC
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Specificité
- Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Human alpha-lactalbumin.
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Réactivité croisée (Details)
- Homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum has not been tested for cross-reactivity.
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Attributs du produit
- The defined antibody specificity is directed to alpha-lactalbumin as tested against human milk. Its presence can also be demonstrated in udder tissues. When tested in immunoelectrophoresis and double radial immunodiffusion against serum no reaction is obtained. To identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of horse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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Purification
- Purified IgG fraction from antiserum
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Immunogène
- Highly purified alpha-lactalbumin isolated from human milk. Alpha-lactalbumin is one of the major proteins in the alpha region after immunoelectrophoresis of human milk. It has been implicated as a component of the enzyme lactose synthetase which catalyzes the formation of lactose from uridine diphosphate galactose and glucose. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Isotype
- IgG
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Indications d'application
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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Buffer
- TRITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
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Agent conservateur
- Without preservative
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- LALBA (Lactalbumin, alpha- (LALBA))
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Autre désignation
- alpha-Lactalbumin
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UniProt
- P00709
Antigène
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