LYZ anticorps (AA 5-220) (FITC)

N° du produit ABIN458127

Cet anticorps anti-LYZ Polyclonal Lapin conjugué à FITC (ABIN458127) détecte spécifiquement LYZ dans ELISA, ICC, IHC (fro) et IFA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour LYZ anticorps (AA 5-220) (FITC) (ABIN458127)

Antigène: LYZ (Lysozyme (LYZ))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LYZ est conjugé à/à la FITC

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Indirect Immunofluorescence Assay (IFA)

Détail du produit anti-LYZ anticorps (FITC)

Épitope: AA 5-220

Fonction: Rabbit anti Human lysozyme, conjugated with FITC

Specificité: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to Human lysozyme.

Réactivité croisée (Details): The antiSerum does not cross-react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. In immunocytochemical and immunohistochemical techniques for the detection of lysozyme at the cellular and subcellular level in appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified

Immunogène: Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low, in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme.Highly purified recombinant human lysozyme was used as immunogen. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. It is reconstituted by adding 1 mL sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

Agent conservateur: Without preservative

Détails sur LYZ

Antigène: LYZ (Lysozyme (LYZ))

Autre désignation: Lysozyme

UniProt: P61626