LYZ anticorps (AA 5-220)

N° du produit ABIN458231

L’anticorps anti-LYZ Polyclonal Lapin est utilisé pour la détection de LYZ dans des échantillons de Humain. Il a été validé pour WB, ELISA, DB et IFA.

Aperçu rapide pour LYZ anticorps (AA 5-220) (ABIN458231)

Antigène: LYZ (Lysozyme (LYZ))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LYZ est non-conjugé

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)

Détail du produit anti-LYZ anticorps

Épitope: AA 5-220

Fonction: Rabbit anti Human lysozyme

Specificité: IgG fraction of polyclonal Rabbit antiSerum to Human lysozyme.

Réactivité croisée (Details): The antiSerum does not cross-react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Attributs du produit: The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.

Purification: Purified IgG fraction from antiserum

Immunogène: Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low, in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme.Highly purified recombinant human lysozyme was used as immunogen. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C.

Détails sur LYZ

Antigène: LYZ (Lysozyme (LYZ))

Autre désignation: Lysozyme

UniProt: P61626