Fibrinogen anticorps

N° du produit ABIN458335

Détail du produit anti-Fibrinogen anticorps

Antigène: Fibrinogen

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibrinogen est non-conjugé

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Specificité: The antiserum does not cross-react with any other component of mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Attributs du produit: Precipitating polyclonal goat antiserum to mouse fibrinogen

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogène: Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immuni-zation procedure.

Isotype: IgG

Information d'application

Indications d'application: In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of fibrinogen in mouse plasma or other body fluids or to determine its concentration.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal mouse plasma in agar-block immunodiffusion titrat

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Agent conservateur: Without preservative

Stock: 4 °C

Références pour anticorps anti-Fibrinogen (ABIN458335)

Miles, Baik, Lighvani, Khaldoyanidi, Varki, Bai, Mueller, Parmer: "Deficiency of plasminogen receptor, Plg-RKT , causes defects in plasminogen binding and inflammatory macrophage recruitment in vivo." dans: Journal of thrombosis and haemostasis : JTH, Vol. 15, Issue 1, pp. 155-162, (2018) (PubMed).

Zang, Gustafsson, Jamalpour, Hong, Genové, Welsh: "Vascular dysfunction and increased metastasis of B16F10 melanomas in Shb deficient mice as compared with their wild type counterparts." dans: BMC cancer, Vol. 15, pp. 234, (2016) (PubMed).

Díaz-Trelles, Scimia, Bushway, Tran, Monosov, Monosov, Peterson, Rentschler, Cabrales, Ruiz-Lozano, Mercola: "Notch-independent RBPJ controls angiogenesis in the adult heart." dans: Nature communications, Vol. 7, pp. 12088, (2016) (PubMed).

Tugues, Roche, Noguer, Orlova, Bhoi, Padhan, Akerud, Honjo, Selvaraju, Mazzone, Tolmachev, Claesson-Welsh: "Histidine-rich glycoprotein uptake and turnover is mediated by mononuclear phagocytes." dans: PLoS ONE, Vol. 9, Issue 9, pp. e107483, (2014) (PubMed).

Lin, Kwong, Warren, Wood, Bhatia: "Nanoparticles that sense thrombin activity as synthetic urinary biomarkers of thrombosis." dans: ACS nano, Vol. 7, Issue 10, pp. 9001-9, (2014) (PubMed).

Détails sur Fibrinogen

Antigène: Fibrinogen

Abstract: Fibrinogen Produits

Synonymes: anticorps fib2, anticorps MGC53620, anticorps fibrinogen, anticorps sb:cb892, anticorps wu:fa55c12, anticorps wu:fb20e08, anticorps wu:fb59b03, anticorps wu:fi39d04, anticorps zgc:92117, anticorps fibrinogen alpha chain, anticorps fibrinogen gamma chain, anticorps fga, anticorps FGA, anticorps LOC698244, anticorps CpipJ_CPIJ010090, anticorps FGG

Sujet: The reactivity of the antiserum is restricted to mouse fibrinogen molecule. In immuno-electrophoresis and radial immunodiffusion, using various antiserum concentrations against normal mouse plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein components or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products