Albumin anticorps (Biotin)

N° du produit ABIN458442

Détail du produit anti-Albumin anticorps (Biotin)

Antigène: Albumin (ALB)

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Albumin est conjugé à/à la Biotin

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificité: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion against several species sera. A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.

Attributs du produit: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to mouse albumin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogène: Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from mouse serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: In immunocytochemical and immunohistochemical use for the detection of albumin at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, in non-isotopic assay methodology (e.g. ELISA) to measure albumin in mouse serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: IgG protein concentration 7.5mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.0. No foreign proteins added.

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détails sur Albumin

Antigène: Albumin (ALB)

Autre désignation: Albumin (ALB Produits)

Synonymes: anticorps PRO0883, anticorps PRO0903, anticorps PRO1341, anticorps ALB, anticorps CSA, anticorps Alb-1, anticorps Alb1, anticorps Albza, anticorps LOC100136344, anticorps alb-a, anticorps alb-b, anticorps albumin, anticorps serum albumin 1, anticorps albumin S homeolog, anticorps ALB, anticorps Alb, anticorps LOC100136344, anticorps alb.S

Sujet: The defined antibody specificity is directed to albumin as tested against mouse sera. In immuno-electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against mouse serum and the purified albumin. cow + chicken - dog - goat - guinea pig ++ hamster + horse + human + monkey + rat ++ sheep - swine +

Pathways: Lipid Metabolism