Albumin anticorps

N° du produit ABIN458740

Cet anticorps Chèvre Polyclonal détecte spécifiquement Albumin dans WB, ELISA, DB et IFA. Il présente une réactivité avec des échantillons de Rat.

Aperçu rapide pour Albumin anticorps (ABIN458740)

Antigène: Albumin (ALB)

Reactivité: Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Albumin est non-conjugé

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)

Détail du produit anti-Albumin anticorps

Fonction: Goat anti Rat albumin

Specificité: Purified IgG fraction of polyclonal Goat antiSerum to Rat albumin

Réactivité croisée: Chien, Cheval, Humain, Singe, Souris, Porc

Aucune reactivité croisée: Poulet, Chévre, Mouton

Réactivité croisée (Details): Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: cow - Goat - Human + Rabbit ± Chicken - Guinea Pig ++ Monkey + Sheep - Dog + Horse + Mouse ++ Swine +

Attributs du produit: The defined antibody specificity is directed to albumin as tested against rat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin. The cytochemical grade of GARa/Alb/7S allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.

Purification: Purified IgG fraction from antiserum

Immunogène: Highly purified albumin isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Information d'application

Indications d'application: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Détails sur Albumin

Antigène: Albumin (ALB)

Autre désignation: Albumin

UniProt: P02770

Pathways: Lipid Metabolism