Albumin anticorps (ALB)

Details for Product anti-ALB Antibody No. ABIN458768
Antigène
Reactivité
Mouton
498
156
153
146
69
65
55
50
47
46
42
39
28
17
14
14
12
4
3
3
2
2
1
1
Hôte
Lapin
726
239
119
45
22
19
5
4
2
Clonalité
Polyclonal
Conjugué
Cet anticorp Albumin est non-conjugé
159
152
109
12
11
8
8
6
6
6
6
6
6
6
2
2
2
2
2
1
1
1
1
1
Application
Immunodiffusion (ID), Immunoelectrophoresis (IEP), Nephelometry (Neph), Radial Immunodiffusion (RID), ELISA
696
656
276
110
108
107
94
68
63
62
58
58
40
27
27
18
18
18
16
12
7
5
3
3
3
2
2
Options
Immunogène Highly purified albumin isolated from sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG
Specificité Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested in double radial immunodiffusion with the following results: bovine ++ chicken - dog ++ goat +++ guinea pig + horse + human + monkey + mouse ± rabbit - rat + swine ++
Attributs du produit Precipitating polyclonal rabbit antiserum to sheep serum albumin
Purification Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Antigène
Autre désignation Serum Albumin (ALB Antibody Extrait)
Sujet The defined antibody specificity is directed to albumin as tested against sheep sera. In immuno- electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against sheep serum and the purified albumin.
Pathways Lipid Metabolism
Indications d'application As precipitating antiserum to identify or measure sheep albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.
Restrictions For Research Use only
Format Lyophilized
Concentration Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:64 when tested against normal sheep serum in agar block immunodiffusion titra
Buffer Delipidated, heat inactivated, lyophilized, stable whole antiserum
Agent conservateur Without preservative
Stock 4 °C/-20 °C
Stockage commentaire The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.