Horseradish Peroxidase (HRP) anticorps

Détails pour le produit réf. ABIN458842
Antigène
Reactivité
Horseradish (Armoracia rusticana)
35
3
2
1
1
Hôte
Souris
22
9
6
2
1
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
2
1
1
Application
ELISA, Western Blotting (WB)
27
27
10
5
5
5
4
3
2
2
2
1
1
1
1
1
Options
Immunogène Highly purified horseradish peroxidase
Clone NI 91
Isotype IgG1 kappa
Specificité This antiserum has not been tested for cross-reactivity.
Attributs du produit Purified monoclonal mouse antibody to horseradish peroxidase
Purification Purified
Antigène
Autre désignation Horseradish Peroxidase
Sujet The reactivity of the antiserum is directed to horseradish as tested in direct binding enzyme immunoassay, ELISA and immunoblotting.
Indications d'application TAs a control in the tests where mouse IgG1 (with irrelevant specificity) should be used to show the presence of that given mouse isotype. In indirect tests as reagent to horseradish peroxidase or to potentiatethe visualisation of a peroxidase labelled conjugate in the appropriate material. As secondary reagent a labelled anti mouse IgG will serve for the detection. This system can be further be potentiated by the use of anti-FITC or anti-TRITC conjugates. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Restrictions For Research Use only
Format Lyophilized
Concentration IgG concentration is 0.4 mg/ml. No foreign proteins added.
Buffer Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
Stock 4 °C/-20 °C
Stockage commentaire The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.