GAPDH anticorps (Glyceraldehyde-3-Phosphate Dehydrogenase)

Details for Product anti-GAPDH Antibody No. ABIN458913
Antigène
Reactivité
Yeast (Saccharomyces cerevisiae)
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254
121
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59
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44
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24
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17
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9
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3
3
2
1
1
1
1
1
1
1
1
1
Hôte
Lapin
374
221
37
26
17
1
Clonalité
Polyclonal
Conjugué
Cet anticorp GAPDH est non-conjugé
50
34
20
7
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5
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2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
1
1
1
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1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
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53
27
25
25
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13
7
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Options
Immunogène Glyceraldehyde-3-phosphate dehydrogenase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificité Cross-reactivities against enzymes of other sources may occur but have not been determined.
Attributs du produit Affinity purified antibodies of polyclonal rabbit antiserum to glyceraldehyde-3-phosphate dehydrogenase from baker’s yeast
Purification Specific polyclonal antibody fractions were prepared by solid phase affinity chromatography.
Antigène
Autre désignation Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH Antibody Extrait)
Sujet The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
Indications d'application This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
Restrictions For Research Use only
Format Lyophilized
Concentration PAb concentration 1 mg/0.5ml. No foreign proteins added.
Buffer Purified hyperimmune rabbit IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran.
Agent conservateur Without preservative
Stock 4 °C/-20 °C
Stockage commentaire The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slow ly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.