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Cholesterol Oxidase anticorps

L’anticorps Lapin Polyclonal anti-Cholesterol Oxidase a été validé pour ELISA, WB et IF. Il convient pour détecter Cholesterol Oxidase dans des échantillons de Nocardia asteroides.
N° du produit ABIN459182

Aperçu rapide pour Cholesterol Oxidase anticorps (ABIN459182)

Antigène

Cholesterol Oxidase (choS)

Reactivité

  • 4
  • 4
  • 2
  • 1
Nocardia asteroides

Hôte

  • 9
  • 2
Lapin

Clonalité

  • 11
Polyclonal

Conjugué

  • 4
  • 3
  • 2
  • 2
Cet anticorp Cholesterol Oxidase est non-conjugé

Application

  • 11
  • 7
  • 2
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Immunofluorescence (IF)
  • Specificité

    Cross-reactivities against enzymes of other sources may occur but have not been determined.

    Attributs du produit

    IgG fraction of polyclonal rabbit antiserum to cholesterol oxidase from Nocardia erythropolis

    Purification

    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

    Immunogène

    Cholesterol oxidase isolated and purified from Nocardia erythropolis. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Indications d'application

    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Concentration

    IgG protein concentration 10 mg/ml. No foreign proteins added.

    Buffer

    Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

    Agent conservateur

    Without preservative

    Stock

    4 °C/-20 °C

    Stockage commentaire

    The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Antigène

    Cholesterol Oxidase (choS)

    Autre désignation

    Cholesterol Oxidase

    Sujet

    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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