beta Amyloid anticorps

N° du produit ABIN459388

Cet anticorps Souris Monoclonal détecte spécifiquement beta Amyloid dans ELISA, IHC et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour beta Amyloid anticorps (ABIN459388)

Antigène: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Amyloid est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: OMAB

Détail du produit anti-beta Amyloid anticorps

Fonction: Anti-Amyloid beta oligomer-specific monoclonal antibody (OMAB)

Réactivité croisée (Details): Human Abeta oligomers only
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Rat

Purification: Affinity purified

Immunogène: Partly aggregated

Isotype: IgM

Information d'application

Indications d'application: Coating antibody at 2 μg/mL (ELISA), 1: 1000 (IF), 1 : 500 (IHC)

Commentaires:

OMAB antibody is a versatile tool within research of Alzheimer's disease. A sandwhich ELISA illustrates its potential regarding its high selectivity towards Aβ oligomers.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Buffer: Affinity purified in PBS pH 7.4.

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: 4 °C

Stockage commentaire: Store lyophilized/reconstituted at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Détails sur beta Amyloid

Antigène: beta Amyloid (Abeta) (Amyloid beta (Abeta))

Autre désignation: Amyloid beta oligomer-specific

Sujet:

Background: Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.

Additional information: OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide.Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.

Poids moléculaire: 4.5 kDa

Pathways: Inflammasome