COX IV anticorps (AA 154-166)

N° du produit ABIN461950

Cet anticorps Chèvre Polyclonal détecte spécifiquement COX IV dans WB, IHC, ELISA et IHC (p). Il présente une réactivité avec des échantillons de Humain et Singe.

Aperçu rapide pour COX IV anticorps (AA 154-166) (ABIN461950)

Antigène: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Reactivité: Humain, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp COX IV est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-COX IV anticorps

Épitope: AA 154-166

Specificité: Human COX4I1. This antibody is expected to recognize COX4I1 (GeneID:1327, MW: 19.6  kDa) & COX4I2 (GeneID:84701, MW:19.6  kDa).

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%) Panda, Dog, Bat, Xenopus (85%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide C-QGLASKWDYEKNE from the C-terminus of human COX4I1 (NP_001852.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%), Panda, Dog, Bat, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:4000), WB (1:4000, 0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur COX IV

Antigène: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Autre désignation: COXIV / COX4

Sujet: Name/Gene ID: COX4I1

Synonyms: COX4I1, COX4-1, COX4, COX IV-1, COXIV

ID gène: 1327

NCBI Accession: NP_001852

UniProt: P13073

Pathways: Proton Transport