Glucose-6-Phosphate Dehydrogenase anticorps (AA 35-506)

N° du produit ABIN462273

Cet anticorps anti-Glucose-6-Phosphate Dehydrogenase est un anticorps Souris Monoclonal détectant Glucose-6-Phosphate Dehydrogenase dans WB, IHC, ELISA et IHC (p). Adapté pour Humain.

Aperçu rapide pour Glucose-6-Phosphate Dehydrogenase anticorps (AA 35-506) (ABIN462273)

Antigène: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Glucose-6-Phosphate Dehydrogenase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AT2F6

Détail du produit anti-Glucose-6-Phosphate Dehydrogenase anticorps

Épitope: AA 35-506

Specificité: Human G6PD (35-506aa)

Purification: Protein G purified

Immunogène: Recombinant human G6PD (35-506aa) purified from E. coli

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of MCF7 (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Détails sur Glucose-6-Phosphate Dehydrogenase

Antigène: Glucose-6-Phosphate Dehydrogenase (G6PD)

Autre désignation: G6PD

Sujet: Name/Gene ID: G6PD

Synonyms: G6PD, G6PD1

ID gène: 2539

UniProt: P11413

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones