Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) anticorps

Détails pour le produit réf. ABIN462364
Antigène
Reactivité
Caenorhabditis elegans (C. elegans), Poulet, Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Singe, Souris, Porc, Plantes, Lapin, Rat (Rattus)
542
355
338
222
207
203
199
193
184
171
124
106
95
86
82
70
48
44
42
27
24
24
23
21
20
18
18
18
16
6
4
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
Hôte
Souris
331
273
8
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
23
11
8
8
8
7
7
7
7
7
7
7
7
7
7
7
7
5
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
510
280
242
201
168
127
96
55
48
45
29
15
11
9
7
3
2
1
1
1
Options
Marque IHC-plus™
Immunogène Human HSP60 produced through recombinant DNA methods in E. coli.

Type of Immunogen: Recombinant protein
Clone LK-2
Isotype IgG1
Specificité Detects an ~60 kD protein, corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, chicken, dog, guinea pig, hamster, monkey, pig, rabbit, plant (spinach), Borrelia, E. coli, helicobacter pylori, M. bovis (Hsp65), Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Yersinia entercolitica, and yeast origins. Deletion mutant analysis indicates that the epitope recognized by LK-2 is located between residues 383-419 of the human Hsp60 sequence (which corresponds to residues 356-393 of the mycobacterial Hsp60 sequence).
Purification Protein G purified
Antigène
Autre désignation HSPD1 / HSP60 (HSPD1 Antibody Extrait)
Sujet Name/Gene ID: HSPD1

Synonyms: HSPD1, 60 kDa chaperonin, CPN60, Heat shock protein 65, Heat shock protein 60, HSP-60, HSP60, HuCHA60, HLD4, HSP65, p60 lymphocyte protein, SPG13, Chaperonin 60, GROEL
ID gène 3329
UniProt P10809
Pathways Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Indications d'application Approved: IHC, IHC-P (10 μg/mL), IP (10 μg/mL), WB (0.25 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
Commentaires

Target Species of Antibody: Human

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS,  pH 7.2, 0.1 mM PMSF, 50 % glycerol
Agent conservateur PMSF
Conseil sur la manipulation avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Stock 4 °C,-20 °C
Stockage commentaire Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
Images (Fournisseur)
Image no. 1 for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (ABIN462364) Anti-HSPD1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, par...
Image no. 2 for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (ABIN462364) Human Adrenal (formalin-fixed, paraffin-embedded) stained with HSPD1 antibody ABIN462...
Image no. 3 for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (ABIN462364) Human Spleen (formalin-fixed, paraffin-embedded) stained with HSPD1 antibody ABIN4623...
Image no. 4 for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (ABIN462364) Human Uterus (formalin-fixed, paraffin-embedded) stained with HSPD1 antibody ABIN4623...