BDNF anticorps (AA 183-194)

N° du produit ABIN462499

Cet anticorps Chèvre Polyclonal détecte spécifiquement BDNF dans IHC et IHC (p). Il présente une réactivité avec des échantillons de Humain, Rat, Souris, Porc, Cheval, Chien, Cobaye, Boeuf (Vache), Hamster et Singe.

Aperçu rapide pour BDNF anticorps (AA 183-194) (ABIN462499)

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivité: Humain, Rat, Souris, Porc, Cheval, Chien, Cobaye, Boeuf (Vache), Hamster, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp BDNF est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-BDNF anticorps

Épitope: AA 183-194

Specificité: Human BDNF. This antibody is expected to recognise all reported isoforms (NP_001700.2, NP_001700.2, NP_733928.1, NP_733929.1, NP_733930.1, NP_733931.1)

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Mouse, Rat, Shrew, Dolphin, Hamster, Elephant, Panda, Bovine, Dog, Cat, Horse, Pig, Guinea pig, Trout, Seabass, Stickleback (100%).

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide ETKCNPMGYTKE from an internal region of human BDNF (NP_001700.2, NP_001700.2, NP_733928.1, NP_733929.1, NP_733930.1, NP_733931.1). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Mouse, Rat, Shrew, Dolphin, Hamster, Elephant, Panda, Bovine, Dog, Cat, Horse, Pig, Guinea pig, Trout, Seabass, Stickleback (100%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur BDNF

Antigène: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Autre désignation: BDNF

Sujet: Name/Gene ID: BDNF
Family: NGF Beta

Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin

ID gène: 627

NCBI Accession: NP_001700, NP_733928, NP_733929, NP_733930, NP_733931

UniProt: P23560

Pathways: Signalisation RTK, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity