IL-15 anticorps

N° du produit ABIN464931

L’anticorps anti-IL-15 Polyclonal Lapin est utilisé pour la détection de IL-15 dans des échantillons de Humain. Il a été validé pour WB, ELISA et Neut.

Aperçu rapide pour IL-15 anticorps (ABIN464931)

Antigène: IL-15 (IL15) (Interleukin 15 (IL15))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL-15 est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-IL-15 anticorps

Specificité: Human IL-15

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human IL-15.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.15 - 0.2 μg/mL), SwELISA, WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 0.5 mg/mL-1 mg/mL with a two hour incubation at room temperature. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-15 (1 ng/mL), a concentration of 0.15-0.20 μg/mL of this antibody is required. ELISA: To detect hIL-15 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-15 (LS-C104326) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-15. Western Blot: To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur IL-15

Antigène: IL-15 (IL15) (Interleukin 15 (IL15))

Autre désignation: IL15

Sujet: Name/Gene ID: IL15
Family: Hormone

Synonyms: IL15, Interleukin-15, IL-15, Interleukin 15

ID gène: 3600

UniProt: P40933

Pathways: Signalistation JAK/STAT, Glycosaminoglycan Metabolic Process