TWEAK anticorps
Aperçu rapide pour TWEAK anticorps (ABIN465064)
Antigène
Voir toutes TWEAK (TNFSF12) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Human TWEAK
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Purification
- Immunoaffinity purified
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Immunogène
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E.coli derived recombinant Human TWEAK.
Type of Immunogen: Cell extract
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Indications d'application
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Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 - 0.2 μg/mL), WB
Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTWEAK (200 ng/mL), a concentration of 0.1-0.2 μg/mL of this antibody is required. ELISA: To detect hTWEAK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TWEAK (LS-C104282) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTWEAK. Western Blot: To detect hTWEAK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTWEAK is 1.5-3 ng/lane, under either reducing or or non-reducing conditions. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Sterile water
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Concentration
- Lot specific
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Buffer
- Lyophilized from PBS, pH 7.2
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Conseil sur la manipulation
- Avoid freeze/thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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