Nerve Growth Factor anticorps

N° du produit ABIN465164

L’anticorps Souris Monoclonal anti-Nerve Growth Factor a été validé pour WB, ELISA et Neut. Il convient pour détecter Nerve Growth Factor dans des échantillons de Humain.

Aperçu rapide pour Nerve Growth Factor anticorps (ABIN465164)

Antigène: Nerve Growth Factor (NGF)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nerve Growth Factor est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-Nerve Growth Factor anticorps

Specificité: Human beta-NGF

Purification: Protein G purified

Immunogène: E.coli derived recombinant Human beta-NGF.

Type of Immunogen: Cell extract

Isotype: IgG2b

Information d'application

Indications d'application: Approved: ELISA (2 - 4 μg/mL), Neut (2.7 - 4 μg/mL), WB (4 - 8 μg/mL)

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (10 ng/mL), a concentration of 2.7-4 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 200 pg/mL of recombinant Human beta-NGF when used with biotinylated antigen affinity purified anti-Human beta-NGF (LS-C104735) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 4-8 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 4-8 ng/lane, under reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur Nerve Growth Factor

Antigène: Nerve Growth Factor (NGF)

Autre désignation: NGF

Sujet: Name/Gene ID: NGF
Family: NGF Beta

Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB

ID gène: 4803

UniProt: P01138

Pathways: Regulation of Cell Size