IL1A anticorps (Biotin)

N° du produit ABIN465287

Cet anticorps Lapin Polyclonal détecte spécifiquement IL1A dans WB, ELISA et Neut. Il présente une réactivité envers Humain.

Aperçu rapide pour IL1A anticorps (Biotin) (ABIN465287)

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IL1A est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Détail du produit anti-IL1A anticorps (Biotin)

Specificité: Human IL-1alpha

Purification: Immunoaffinity purified

Immunogène: E.coli derived recombinant Human IL-1alpha.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hIL-1alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-1alpha. To detect hIL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-1alpha (LS-C104630) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-1alpha. Western Blot: To detect hIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur IL1A

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Autre désignation: IL1A / IL1 Alpha

Sujet: Name/Gene ID: IL1A
Family: IL-1

Synonyms: IL1A, Hematopoietin-1, IL-1 alpha, IL1, IL1-ALPHA, IL1 Alpha, Interleukin-1 alpha, IL-1A, IL1F1, Pro-interleukin-1-alpha, Preinterleukin 1 alpha, Interleukin 1, alpha

ID gène: 3552

UniProt: P01583

Pathways: Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints