WISP2 anticorps (Biotin)

N° du produit ABIN465432

L’anticorps anti-WISP2 Polyclonal Lapin est utilisé pour la détection de WISP2 dans des échantillons de Humain. Il a été validé pour WB et ELISA.

Aperçu rapide pour WISP2 anticorps (Biotin) (ABIN465432)

Antigène: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp WISP2 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-WISP2 anticorps (Biotin)

Specificité: Human CTGFL/WISP-2

Purification: Affinity purified

Immunogène: E.coli derived recombinant Human CTGFL/WISP-2.

Type of Immunogen: Cell extract

Information d'application

Indications d'application: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hCTGFL/WISP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGFL/WISP-2. To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human CTGFL/WISP-2 (LS-C104572) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 50 μL sterile PBS containing 0.1 % BSA.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Conseil sur la manipulation: Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Détails sur WISP2

Antigène: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Autre désignation: WISP2

Sujet: Name/Gene ID: WISP2

Synonyms: WISP2, CCN5, CCN family member 5, CTGFL, CT58, CTGF-L, WISP-2

ID gène: 8839

UniProt: O76076

Pathways: Signalisation WNT, Growth Factor Binding