S100A8/A9 Complex (Calprotectin) anticorps

N° du produit ABIN481651

Cet anticorps Souris Monoclonal détecte spécifiquement S100A8/A9 Complex (Calprotectin) dans IHC, ELISA et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour S100A8/A9 Complex (Calprotectin) anticorps (ABIN481651)

Antigène: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp S100A8/A9 Complex (Calprotectin) est non-conjugé

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 27E10

Détail du produit anti-S100A8/A9 Complex (Calprotectin) anticorps

Specificité: The antigen is MRP8/14 (calprotectin), the epitope involves parts of both subunits MRP8 and MRP14. Antigen distribution: Isolated cells: Monocytes carry the antigen both on the surface and intracellularly, granulocytes exhibit it only intracellularly. Up to 80 % of monocytes in early cultures (24- 48h) are positive. No reaction has been seen with lymphocytes or platelets. Tissue sections: The antigen is found in macrophages in the red pulp of the spleen and liver. It is strongly expressed in macrophages from acute inflamed tissues (peridontitis, contact eczema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen. It is normally absent on resident mononuclear phagocytes in healthy tissues (skin, gut, thymus). Specificity: Human: subpopulation of macrophages, monocytes and granulocytes, peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Other: subpopulation of macrophages of rhesus monkey and bovine tissues. The antibody does not react with swine tissues.

Purification: Affinity purified

Immunogène: Cultured human monocytes

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-P (1 μg/mL)

Usage: For IHC-P, Proteinase K pretreatment is recommended for antigen retrieval.

Not recommended for: IHC-Fr

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 5 mg/mL BSA, and 0.05 % Kathon CG

Agent conservateur: Kathon CG

Conseil sur la manipulation: Avoid freeze/thaw cycles. Store undiluted.

Stock: 4 °C,-20 °C

Stockage commentaire: Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

Détails sur S100A8/A9 Complex (Calprotectin)

Antigène: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Autre désignation: Calprotectin

Pathways: S100 Proteins