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Toc75 anticorps

Toc75 Reactivité: Pisum sativum, Synechococcus sp., Thermosynechococcus, Synechocystis FACS, IL, WB Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN488514
  • Antigène Tous les produits Toc75
    Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))
    Reactivité
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Pisum sativum, Synechococcus sp., Thermosynechococcus, Synechocystis
    Hôte
    • 1
    Lapin
    Clonalité
    • 1
    Polyclonal
    Conjugué
    • 1
    Cet anticorp Toc75 est non-conjugé
    Application
    Flow Cytometry (FACS), Immunolocalization (IL), Western Blotting (WB)
    Réactivité croisée (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    Homologie
    Ricinus communis, Vitis vinifera, Populus trichocarpa
    Attributs du produit
    Expected / apparent Molecular Weight of the Antigene: 88 / 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
    Purification
    serum
    Immunogène
    psTOC75, Predicted POTRA Domain #1, Amino acids, 158-241, Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715
  • Indications d'application
    1: 2000 - 1: 25 000, 1: 100 000 (WB), 1: 500 (FACS). 1: 100 (IL)
    Commentaires

    antibody detects Toc75 POTRA domain 1 as purified protein, in chloroplast fraction and in crude envelope fraction

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 μL of sterile water
    Conseil sur la manipulation
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Antigène
    Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))
    Abstract
    Toc75 Produits
    Sujet
    OEP 75 or Toc75, Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446), In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87, Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane, YaeT, outer membrane protein assembly complex, (TIGR03303), FhaC, Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]
    Poids moléculaire
    expected: 88 kDa, apparent: 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)
    ID gène
    158241
    UniProt
    Q43715
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