Toc75 anticorps (Chloroplastic, Domain 3)

N° du produit ABIN5684021

Détail du produit anti-Toc75 anticorps

Antigène: Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))

Épitope: Chloroplastic, Domain 3

Reactivité: Pisum sativum, Synechococcus sp., Thermosynechococcus, Nicotiana tabacum, Spinach, Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Toc75 est non-conjugé

Application: Flow Cytometry (FACS), Immunolocalization (IL), Western Blotting (WB)

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 88 / 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)

Purification: serum

Immunogène: psTOC75, Predicted POTRA Domain #3, Expressed and purified in E. coli using the Impact System from NEB. Peptide confirmed by MALDI. Q43715

Information d'application

Indications d'application: Recommended Dilution: 1 : 2000 - 1 : 25 000, 1 : 100 000 (WB), 1 : 500 (FACS). 1 : 100 (LSCM).

Commentaires:

antibody detects Toc75 POTRA domain 3 as purified protein, in chloroplast fraction and in crude envelope fraction

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Détails sur Toc75

Antigène: Toc75 (Chloroplast Outer Envelope Membrane Translocon Complex Oep75 Protein (Toc75))

Abstract: Toc75 Produits

Sujet: AGI Code: At3g46740
OEP 75 or Toc75, Chloroplast outer envelope membrane protein from Pisum sativum (pea), Predicted to contain 3 POTRA domains at N-terminus. Believed to be the protein conducting channel of the Toc translocon and assembles as an 18 stranded ß-barrel (EMBO J. (1995) 14:11, 2436-2446), In Arabidopsis there are five members of this Family Toc75 (I-V), atToc75III is most closely related to psToc75. Additionally, it is structurally related to members of the bacterial surface antigen super-family including: OMA87, Outer membrane protein/protective antigen, (COG4775, COG4775) [Cell envelope biogenesis, outer membrane, YaeT, outer membrane protein assembly complex, (TIGR03303), FhaC, Hemolysin activation/secretion protein (COG2831) [Intracellular trafficking and secretion]

Poids moléculaire: expected: 88 kDa, apparent: 75 kDa (ocassionally a processing intermediate at 78 kDa is observed)

UniProt: Q43715