PLAP anticorps (C-Term)

N° du produit ABIN4886453

L’anticorps Lapin Polyclonal anti-PLAP a été validé pour WB, IHC, FACS, IF et ICC. Il convient pour détecter PLAP dans des échantillons de Humain.

Aperçu rapide pour PLAP anticorps (C-Term) (ABIN4886453)

Antigène: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PLAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-PLAP anticorps

Épitope: AA 403-433, C-Term

Fonction: Anti-Placental alkaline phosphatase (PLAP)/ALPP Antibody Picoband®

Séquence: KARDRKAYTV LLYGNGPGYV LKDGARPDVT E

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Placental alkaline phosphatase (PLAP)/ALPP Antibody Picoband® (ABIN4886453). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human ALPP.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: ALPP alkaline phosphatase, placental (Regan isozyme)". 2. Henthorn PS, Knoll BJ, Raducha M, Rothblum KN, Slaughter C, Weiss M, Lafferty MA, Fischer T, Harris H (Sep 1986). "Products of two common alleles at the locus for human placental alkaline phosphatase differ by seven amino acids". Proc Natl Acad Sci U S A. 83 (15): 5597-601. 3. Kam W, Clauser E, Kim YS, Kan YW, Rutter WJ (Feb 1986). "Cloning, sequencing, and chromosomal localization of human term placental alkaline phosphatase cDNA". Proc Natl Acad Sci U S A. 82 (24): 8715-9.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PLAP

Antigène: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Autre désignation: ALPP

Sujet:

Synonyms: Alkaline phosphatase, placental type,3.1.3.1,Alkaline phosphatase Regan isozyme,Placental alkaline phosphatase 1,PLAP-1,ALPP,PLAP,

Tissue Specificity: Detected in placenta (at protein level). .

Background: Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells.

Poids moléculaire: 70 kDa

ID gène: 250

UniProt: P05187