KDM5B anticorps (Middle Region)

N° du produit ABIN4886648

L’anticorps Lapin Polyclonal anti-KDM5B a été validé pour WB, IHC, FACS, IF et ICC. Il convient pour détecter KDM5B dans des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour KDM5B anticorps (Middle Region) (ABIN4886648)

Antigène: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp KDM5B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-KDM5B anticorps

Épitope: AA 641-685, Middle Region

Fonction: Anti-KDM5B/PLU1/Jarid1B Antibody Picoband®

Séquence: DVLDVVVAST VQKDMAIMIE DEKALRETVR KLGVIDSERM DFE

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-KDM5B/PLU1/Jarid1B Antibody Picoband® (ABIN4886648). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence in the middle region of human KDM5B, identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Lahoud MH, Ristevski S, Venter DJ, Jermiin LS, Bertoncello I, Zavarsek S, Hasthorpe S, Drago J, de Kretser D, Hertzog PJ, Kola I (August 2001). "Gene targeting of Desrt, a novel ARID class DNA-binding protein, causes growth retardation and abnormal development of reproductive organs". Genome Research 11(8): 1327-34. 2. Zhu L, Hu J, Lin D, Whitson R, Itakura K, Chen Y (August 2001). "Dynamics of the Mrf-2 DNA-binding domain free and in complex with DNA". Biochemistry 40 (31): 9142-50.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur KDM5B

Antigène: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Autre désignation: KDM5B

Sujet:

Synonyms: Lysine-specific demethylase 5B,1.14.11.-,Cancer/testis antigen 31,CT31,Histone demethylase JARID1B,Jumonji/ARID domain-containing protein 1B,PLU-1,Retinoblastoma-binding protein 2 homolog 1,RBP2-H1,KDM5B,JARID1B, PLU1, RBBP2H1,

Tissue Specificity: Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level). .

Background: Lysine-specific demethylase 5B, also known as histone demethylase JARID1B, is a demethylase enzyme that in humans is encoded by the KDM5B gene. This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing resultsi n multiple transcript variants.

Poids moléculaire: 180 kDa

ID gène: 10765

Pathways: L'effet Warburg