Lactate Dehydrogenase A anticorps (AA 2-106)

N° du produit ABIN4886657

L’anticorps Lapin Polyclonal anti-Lactate Dehydrogenase A a été validé pour WB, IHC, IF, ICC et FACS. Il convient pour détecter Lactate Dehydrogenase A dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Lactate Dehydrogenase A anticorps (AA 2-106) (ABIN4886657)

Antigène: Lactate Dehydrogenase A (LDHA)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Lactate Dehydrogenase A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-Lactate Dehydrogenase A anticorps

Épitope: AA 2-106

Fonction: Anti-LDHA Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-LDHA Antibody Picoband® (ABIN4886657). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human LDHA recombinant protein (Position: A2-R106). Human LDHA shares 94.3% amino acid (aa) sequence identity with both mouse and rat LDHA.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Chung FZ, Tsujibo H, Bhattacharyya U, Sharief FS, Li SS (November 1985). "Genomic organization of human lactate dehydrogenase-A gene". Biochem. J. 231 (3): 537-41. 2. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925-7.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Lactate Dehydrogenase A

Antigène: Lactate Dehydrogenase A (LDHA)

Autre désignation: LDHA

Sujet:

Synonyms: L-lactate dehydrogenase A chain,LDH-A,1.1.1.27,Cell proliferation-inducing gene 19 protein,LDH muscle subunit,LDH-M,Renal carcinoma antigen NY-REN-59,LDHA,PIG19,

Tissue Specificity: Low levels in brain. Very weak expression found in heart and muscle. .

Background: Lactate dehydrogenase A, also known as LDHA, is an enzyme which in humans is encoded by the LDHA gene. The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Poids moléculaire: 37 kDa

ID gène: 3939

UniProt: P00338

Pathways: L'effet Warburg