Nucleolin anticorps (pThr84)

N° du produit ABIN4889383

Cet anticorps Lapin Polyclonal détecte spécifiquement Nucleolin dans WB, IF et IP. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour Nucleolin anticorps (pThr84) (ABIN4889383)

Antigène: Nucleolin (NCL)

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nucleolin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Détail du produit anti-Nucleolin anticorps

Épitope: pThr84

Specificité: Reacts with Nucleolin with phoshothreonine 84 (pT84) from human and mouse

Réactivité croisée (Details): Cross reacts with phoshothreonine 106 (pT106). Do not cross react with phoshothreonine 76 & 92 (pT76 and pT92). The detection of P-NCL can be done during mitosis or in G2 phase, no signal in G1S phase.

Purification: Purified (protein A)

Immunogène: Synthetic peptide of human Nucleolin with a phoshothreonine at position 84

Information d'application

Indications d'application:

Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
- WB : 1/200 to 1/2000 - IF: 1/200 to 1/1000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Concentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M

Stock: 4 °C/-20 °C

Stockage commentaire: Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Date de péremption: 6 months

Détails sur Nucleolin

Antigène: Nucleolin (NCL)

Autre désignation: Nucleolin

Sujet: Ucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

ID gène: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization