Histone 3 anticorps (H3K27me3)

N° du produit ABIN4889658

Cet anticorps Souris Monoclonal détecte spécifiquement Histone 3 dans WB, IF, ChIP et ICC. Il présente une réactivité avec des échantillons de Humain. Il a été cité dans 4+ publications.

Aperçu rapide pour Histone 3 anticorps (H3K27me3) (ABIN4889658)

Antigène: Histone 3 (H3) (Histone H3 (H3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Histone 3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC)

Clone: 7B11

Détail du produit anti-Histone 3 anticorps

Épitope: H3K27me3

Fonction: Histone H3K27me2me3 antibody (mAb) (Clone 7B11)

Purification: Protein A Chromatography

Immunogène: This Histone H3 di/trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3.

Isotype: IgG

Information d'application

Indications d'application: ICC/IF: 0.5 - 2 μg/mL dilution WB*: 0.5 - 2 μg/mL dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot. This antibody has been published for use in ChIP, but has not been validated by Active Motif for this application. ChIP-validated antibodies are available for this target: Catalog No. 39535.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Purified IgG in 10 mM sodium phosphate ( pH 7.5), 150 mM NaCl, 30 % glycerol, 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-Histone 3 (ABIN4889658)

Nolan, Franco, Hance, Hayward, Isaacs: "Tumor-secreted Hsp90 subverts polycomb function to drive prostate tumor growth and invasion." dans: The Journal of biological chemistry, Vol. 290, Issue 13, pp. 8271-82, (2015) (PubMed).

Papazyan, Voronina, Chapman, Luperchio, Gilbert, Meier, Mackintosh, Shabanowitz, Tackett, Reddy, Coyne, Hunt, Liu, Taverna: "Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis." dans: eLife, Vol. 3, pp. e02996, (2014) (PubMed).

Ivanov, Pawlikowski, Manoharan, van Tuyn, Nelson, Rai, Shah, Hewitt, Korolchuk, Passos, Wu, Berger, Adams: "Lysosome-mediated processing of chromatin in senescence." dans: The Journal of cell biology, Vol. 202, Issue 1, pp. 129-43, (2013) (PubMed).

Rada-Iglesias, Bajpai, Prescott, Brugmann, Swigut, Wysocka: "Epigenomic annotation of enhancers predicts transcriptional regulators of human neural crest." dans: Cell stem cell, Vol. 11, Issue 5, pp. 633-48, (2012) (PubMed).

Détails sur Histone 3

Antigène: Histone 3 (H3) (Histone H3 (H3))

Autre désignation: Histone H3

Sujet: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

Poids moléculaire: 17 kDa

ID gène: 3020

NCBI Accession: NP_003522