ATG3 anticorps

N° du produit ABIN4906359

Cet anticorps anti-ATG3 est un anticorps Souris Monoclonal détectant ATG3 dans WB et IHC (p). Adapté pour Humain et Souris.

Aperçu rapide pour ATG3 anticorps (ABIN4906359)

Antigène: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ATG3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7C2

Détail du produit anti-ATG3 anticorps

Réactivité croisée: Humain, Souris

Purification: Purified by Protein G.

Immunogène: This ATG3 antibody is generated from a mouse immunized with recombinant protein of human ATG3.

Isotype: IgG1

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur ATG3

Antigène: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Autre désignation: ATG3

Sujet:

Synonyms: APG3, APG3L, PC3-96, APG3-LIKE, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, hApg3, Protein PC3-96, ATG3

Background: E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

ID gène: 64422

UniProt: Q9NT62

Pathways: Autophagy