RPA2 anticorps

N° du produit ABIN4913599

Cet anticorps Souris Monoclonal détecte spécifiquement RPA2 dans WB et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour RPA2 anticorps (ABIN4913599)

Antigène: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RPA2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5C3

Détail du produit anti-RPA2 anticorps

Réactivité croisée: Humain

Purification: Purified by Protein G.

Immunogène: This RFA2 antibody is generated from a mouse immunized with a recombinant protein of human RFA2.

Isotype: IgG1

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C for 12 months.

Date de péremption: 12 months

Détails sur RPA2

Antigène: RPA2 (Replication Protein A2, 32kDa (RPA2))

Autre désignation: RFA2

Sujet:

Synonyms: REPA2, RPA32, RP-A p32, RP-A p34, Replication protein A 32 kDa subunit, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RPA2, RPA34

Background: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

ID gène: 6118

UniProt: P15927

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA