Topoisomerase I anticorps

N° du produit ABIN4914765

Cet anticorps anti-Topoisomerase I est un anticorps Souris Monoclonal détectant Topoisomerase I dans WB et FACS. Adapté pour Humain et Rat.

Aperçu rapide pour Topoisomerase I anticorps (ABIN4914765)

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase I est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 1C6

Détail du produit anti-Topoisomerase I anticorps

Réactivité croisée: Humain, Rat

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogène: This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.

Isotype: IgG1

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur Topoisomerase I

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Autre désignation: TOP1

Sujet:

Synonyms: TOPI, DNA topoisomerase 1, DNA topoisomerase I, TOP1

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

ID gène: 7150

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance