CAMK2A anticorps (pThr286)

N° du produit ABIN492420

Cet anticorps anti-CAMK2A Monoclonal Souris (Clone 22B1) (ABIN492420) détecte spécifiquement CAMK2A dans WB, IF, IP, EIA et IHC (fro). L’anticorps est réactif avec des échantillons de Humain et Rat.

Aperçu rapide pour CAMK2A anticorps (pThr286) (ABIN492420)

Antigène: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CAMK2A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 22B1

Détail du produit anti-CAMK2A anticorps

Épitope: pThr286

Réactivité croisée (Details): Species reactivity (tested):Rat, Human.

Attributs du produit: Synonyms: CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-IIsubunit alpha, Calcium/calmodulin-dependent protein kinase type II subunit alpha

Purification: Protein G Chromatography.

Immunogène: Synthetic peptide

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Immunflourescence: 10 μg/mL. Immunoprecipitaion. ImmunohistochemistryWestern blot: 1 μg/mL, A band of approx. 50 kDa is detected.1 μg/mL of CAMKII was sufficient for detection of 0.2 μg CAMKII by colorimetric immunoblot. (Ref.13)

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4 containing 0.09 % Sodium Azide in 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CAMK2A

Antigène: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Autre désignation: CAMK2A

Sujet: CaMKII is an important member of calcium/calmodulinactivated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca 2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).Synonyms: CAMKA, CaM kinase II subunit alpha, CaM-kinase II alpha chain, CaMK-II subunit alpha, Calcium/calmodulin-dependent protein kinase type II subunit alpha, KIAA0968

ID gène: 25400

UniProt: P11275

Pathways: Signalisation WNT, Interferon-gamma Pathway, Myometrial Relaxation and Contraction