Endonuclease G anticorps (AA 51-140)

N° du produit ABIN492521

L’anticorps Souris Monoclonal anti-Endonuclease G a été validé pour WB et EIA. Il convient pour détecter Endonuclease G dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour Endonuclease G anticorps (AA 51-140) (ABIN492521)

Antigène: Endonuclease G (ENDOG)

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Endonuclease G est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 7G1C10

Détail du produit anti-Endonuclease G anticorps

Épitope: AA 51-140

Specificité: Recognizes Endonuclease G (EndoG)

Attributs du produit: Synonyms: Endo-G, Endonuclease G mitochondrial

Purification: Immunoaffinity Chromotography.

Immunogène: Recombinant protein corresponding to amino acids 51–140 of Human EndoG.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western Blot: EndoG antibody can be used for detection of EndoG at 2.5-5 μg/mL. Positive Controls: HepG2 cell lysate, EndoG Recombinant Protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Endonuclease G

Antigène: Endonuclease G (ENDOG)

Autre désignation: Endonuclease G

Sujet: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.Synonyms: ENDOG

ID gène: 2021

UniProt: Q14249

Pathways: Apoptose