LC3B anticorps (N-Term)
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- Antigène Voir toutes LC3B (MAP1LC3B) Anticorps
- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
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Épitope
- N-Term
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Reactivité
- Humain, Souris, Rat, Hamster, Chien
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp LC3B est non-conjugé
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Application
- Western Blotting (WB), Immunofluorescence (IF)
- Specificité
- This antibody specifically recognizes both forms of endogenous LC3, the cytoplasmic LC3-I (18 kDa) as well as the lipidated form generated during autophagosome and autophagolysosome formation: LC3-II (16 kDa). Immunocytochemical staining of cells with LC3 antibody Cat.-No AM20212PU-N (Clone 5F10) reveals the specific punctate distribution of endogenous LC3-II as a hallmark of autophagic activity.
- Attributs du produit
- Synonyms: MAP1LC3B, MAP1A/MAP1B, Map1lc3b, Map1alc3, Map1lc3, Microtubule-associated protein1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B,Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A /1B light chain 3 B
- Purification
- Subsequent Ultrafiltration and Size Exclusion Chromatography
- Ingrédients
- incl. positive Control
- Immunogène
- Synthetic peptide hemocyanin conjugated derived from the N-terminus of LC3-B
- Clone
- 5F10
- Isotype
- IgG1
- Top Product
- Discover our top product MAP1LC3B Anticorps primaire
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- Indications d'application
- Immunoblotting: 0.5 μg/mL for HRPO/ECL detectionRecommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. We strongly recommend to use PVDF membranes for immunoblot analysis. Immunocytochemistry: Use at 1-10 μg/mL (Paraformaldehyd/Methanol fixation). Included Positive Control: Cell lysate from untreated Neuro 2A (See Protocols).
- Protocole
- Positive Control: Cell lysate from untreated Neuro 2A cells, brain endothelioma (Mouse)Format: Lyophilized cell lysate from serum starved Neuro 2A. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1 mL H2O (15 min, RT)
- Buffer
- 1 mLPBS, 0.09 % Sodium Azide, PEG and Sucrose
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C/-80 °C
- Stockage commentaire
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Date de péremption
- 12 months
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- Antigène
- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
- Autre désignation
- LC3B (MAP1LC3B Produits)
- Synonymes
- anticorps ATG8F, anticorps LC3B, anticorps MAP1A/1BLC3, anticorps MAP1LC3B-a, anticorps Map1lc3, anticorps Mpl3, anticorps zbs559, anticorps 1010001C15Rik, anticorps Atg8, anticorps LC3b, anticorps zgc:56434, anticorps wu:fb60g11, anticorps map1lc3b, anticorps MGC76283, anticorps MAP1LC3B, anticorps microtubule associated protein 1 light chain 3 beta, anticorps microtubule-associated protein 1 light chain 3 beta, anticorps microtubule associated protein 1 light chain 3 beta L homeolog, anticorps MAP1LC3B, anticorps Map1lc3b, anticorps map1lc3b, anticorps map1lc3b.L
- Sujet
- Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (microtubule-associated protein1 light chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.Synonyms: Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A / 1B light chain 3 B, MAP1A/MAP1B, MAP1LC3B, Map1alc3, Map1lc3, Map1lc3b, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B
- Poids moléculaire
- 18 kDa (LC3-I), 16 kDa (LC-II)
- ID gène
- 81631
- UniProt
- Q9GZQ8
- Pathways
- Autophagy
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