LC3B anticorps (Internal Region)

N° du produit ABIN492616

Cet anticorps Souris Monoclonal détecte spécifiquement LC3B dans WB et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour LC3B anticorps (Internal Region) (ABIN492616)

Antigène: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp LC3B est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 5H3

Détail du produit anti-LC3B anticorps

Épitope: Internal Region

Specificité: Recognizes LC3A, LC3B and LC3C

Attributs du produit: Synonyms: LC3A, LC3B, LC3C, Microtubule-associated protein 1 light chain 3, Autophagy-relatedprotein LC3, Autophagy-related ubiquitin-like modifier LC3

Purification: Purified from Serum-Free Cell Culture Supernatant by Subsequent Ultrafiltration and Size Exclusion Chromatography.

Ingrédients: incl. positive Control

Immunogène: Synthetic peptide conjugated to hemocyaninEpitope: Internal sequence that is identical in LC3A, LC3B and LC3C

Isotype: IgG1

Information d'application

Indications d'application: Immunoblotting: 0.5 μg/mL for HRPO/ECL detectionRecommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. We strongly recommend to use PVDF membranes for immunoblot analysis. Immunocytochemistry: Use at 1-10 μg/mL (Paraformaldehyd/Methanol fixation). Included Positive Control: Cell lysate from untreated Neuro 2A (See Protocols).

Protocole: Positive Control: Cell lysate from untreated Neuro 2A cells, brain endothelioma (Mouse)Format: Lyophilized cell lysate from serum starved Neuro 2A. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS with 0.09 % Sodium Azide, PEG, Sucrose and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C/-80 °C

Stockage commentaire: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur LC3B

Antigène: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Autre désignation: LC3B

Sujet: Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (microtubule-associated protein1 light chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.Synonyms: Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A / 1B light chain 3 B, MAP1A/MAP1B, MAP1LC3B, Map1alc3, Map1lc3, Map1lc3b, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B

ID gène: 81631

UniProt: Q9GZQ8

Pathways: Autophagy