RAPTOR anticorps
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- Antigène Voir toutes RAPTOR Anticorps
- RAPTOR
- Reactivité
- Humain, Souris, Rat, Chien
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp RAPTOR est non-conjugé
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Application
- Western Blotting (WB)
- Specificité
- Recognizes Raptor.
- Attributs du produit
- Synonyms: RPTOR, KIAA1303, Regulatory-associated protein of mTOR, P150 target of rapamycinTOR-scaffold protein
- Purification
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
- Ingrédients
- incl. positive Control
- Immunogène
- Peptide conjugated to hemocyanin derived from Raptor Sequence.
- Clone
- 1-00E-011
- Isotype
- IgG1
- Top Product
- Discover our top product RAPTOR Anticorps primaire
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- Indications d'application
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Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HeLa cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocole
- Positive Control Provided. Cell lysate from untreated Hela cellsDescription: Cell lysate from untreated HeLa cells, epitheloid cervical carcinoma (Human)Format: Lyophilized cell lysate from serum starved HeLa cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
- Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1 mL H2O (15 min, RT).
- Buffer
- 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
- Date de péremption
- 12 months
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- Antigène
- RAPTOR
- Autre désignation
- RAPTOR (RAPTOR Produits)
- Synonymes
- anticorps KOG1, anticorps Mip1, anticorps 4932417H02Rik, anticorps Rap, anticorps Raptor, anticorps mKIAA1303, anticorps RGD1311784, anticorps regulatory associated protein of MTOR complex 1, anticorps regulatory associated protein of MTOR, complex 1, anticorps RPTOR, anticorps Rptor
- Sujet
- The mTOR pathway (mammalian target of rapamycin) coordinates nutrient signals with growth factor dependent signaling. Recent data revealed that mTOR is organized in two independent protein complexes: The complex containing mTOR, GbL and RAPTOR is target of rapamycin while the complex containing mTOR, GbL and RICTOR is insensitive to rapamycin. The mTOR /RICTOR complex phosphorylates PKBalpha/akt1 at Serine 473Synonyms: KIAA1303, P150 target of rapamycin TOR-scaffold protein, RPTOR, Regulatory-associated protein of mTOR
- ID gène
- 57521
- UniProt
- Q8N122
- Pathways
- L'effet Warburg
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