Iba1 anticorps (C-Term)

N° du produit ABIN492698

L’anticorps Chèvre Polyclonal anti-Iba1 a été validé pour WB, IHC (p) et EIA. Il convient pour détecter Iba1 dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour Iba1 anticorps (C-Term) (ABIN492698)

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivité: Humain, Rat, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Iba1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-Iba1 anticorps

Épitope: C-Term

Specificité: This antibody recognizes Allograft Inflammatory Factor-1 (AIF1).

Réactivité croisée (Details): Species reactivity (expected):Mouse, Bovine, Goat, Hamster, Rabbit, Pig, Horse, MonkeySpecies reactivity (tested):Human and Rat

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide (C-TGPPAKKAISELP) from C-terminus of Human AIF1

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/128000. Western blot: 1-3 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This antibody was validated for use in Immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by AlkalinePhosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody in aliquots at-20 °C.

Détails sur Iba1

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Autre désignation: AIF1

Sujet: Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

ID gène: 199

NCBI Accession: NP_001614

UniProt: P55008

Pathways: Smooth Muscle Cell Migration