Recombinant Keratin Acidic (AE1) anticorps

N° du produit ABIN4949248

L’anticorps Lapin Monoclonal anti- a été validé pour IF, WB, FACS et IHC (p). Il convient pour détecter dans des échantillons de Humain.

Aperçu rapide pour Recombinant Keratin Acidic (AE1) anticorps (ABIN4949248)

Antigène: Keratin Acidic (AE1)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: KRTL-1577R

Détail du produit

Purification: Protein A affinity chromatography

Immunogène: An amino acid sequence common to acidic/type I keratins (1) was used as the immunogen for the recombinant Acidic Cytokeratin antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: Titering of the recombinant Acidic Cytokeratin antibody may be required for optimal performance.

1. This antibody will detect cytokeratins -10, -14, -15, -16, -19.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
3. Required HIER: boil tissue sections in 10  mM citrate buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.\. Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (3),Prediluted IHC only format: incubate for 30 min at RT (2),FACS: 0.5-1 μg/million cells in 0.1ml,WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant Acidic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détail du antigène

Antigène: Keratin Acidic (AE1)

Autre désignation: Acidic Cytokeratin

Sujet: There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]