Recombinant CD34 anticorps

N° du produit ABIN4949293

L’anticorps anti-CD34 Monoclonal Lapin est utilisé pour la détection de CD34 dans des échantillons de Humain et Rat. Il a été validé pour FACS, WB, IF et IHC (p).

Aperçu rapide pour Recombinant CD34 anticorps (ABIN4949293)

Antigène: CD34

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HPCA1-1806R

Détail du produit anti-CD34 anticorps

Purification: Protein A affinity chromatography

Immunogène: Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: Optimal dilution of the recombinant CD34 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 2-4 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant CD34 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD34

Antigène: CD34

Autre désignation: CD34

Sujet: This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120  kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi s sarcoma, but shows low specificity.