Recombinant CD44v4 anticorps

N° du produit ABIN4949301

Cet anticorps Lapin Monoclonal détecte spécifiquement CD44v4 dans FACS, IF et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant CD44v4 anticorps (ABIN4949301)

Antigène: CD44v4

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CD44v4-1700R

Détail du produit

Purification: Protein A affinity chromatography

Immunogène: An amino acid sequence from the variant 4 domain of CD44 was used as the immunogen for the recombinant CD44v4 antibody.

Isotype: IgG kappa

Information d'application

Indications d'application: Titering of the recombinant CD44v4 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the recombinant CD44v4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détail du antigène

Antigène: CD44v4

Sujet: This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 Molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. It is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.