Recombinant CD44v4 anticorps (CF®647)

N° du produit ABIN6165393

Détail du produit

Antigène: CD44v4

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Fonction: Recombinant Rabbit Monoclonal anti-CD44v4 (CD44v4/1700R), CF647 Conjugate

Attributs du produit: This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 Molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant fragment corresponding to the v4 domain of human CD44 (exact sequence is proprietary)

Clone: CD44v4-1700R

Isotype: IgG kappa

Information d'application

Indications d'application: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

Cervix or Tongue Squamous Cell Carcinoma

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Protect from light

Détail du antigène

Antigène: CD44v4

Poids moléculaire: 80-95 kDa

ID gène: 960, 502328

UniProt: P16070