CD35 anticorps

N° du produit ABIN4949510

Cet anticorps anti-CD35 est un anticorps Souris Monoclonal détectant CD35 dans FACS, IF et IHC (p). Adapté pour Humain, Babouin et Singe.

Aperçu rapide pour CD35 anticorps (ABIN4949510)

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivité: Humain, Babouin, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD35 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CR1-802

Détail du produit anti-CD35 anticorps

Purification: Protein G affinity chromatography

Immunogène: Recombinant human protein was used as the immunogen for the CD35 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the CD35 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the CD35 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur CD35

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Autre désignation: CD35

Sujet: Recognizes a protein of 210-220  kDa, which is identified as the complement receptor 1 (CR1)/CD35. This mAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This mAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity