GJB1 anticorps (Cytoplasmic Loop)

N° du produit ABIN4949578

L’anticorps Souris Monoclonal anti-GJB1 a été validé pour WB, IF, FACS et IHC (fro). Il convient pour détecter GJB1 dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour GJB1 anticorps (Cytoplasmic Loop) (ABIN4949578)

Antigène: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GJB1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: M12-13

Détail du produit anti-GJB1 anticorps

Épitope: Cytoplasmic Loop

Purification: Protein G affinity chromatography

Immunogène: Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Connexin 32 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur GJB1

Antigène: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Autre désignation: Connexin 32 / GJB1

Sujet: This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

Pathways: Cell-Cell Junction Organization